EK382HS

Rat TNF-α High Sensitivity ELISA Kit檢測試劑盒（酶聯免疫吸附法）

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¥2,000.00 – ¥3,400.00

  • 分子靶點：TNF, TNFA, TNFSF2
  • 種屬：大鼠 (Rat)
  • 樣本類型：血清，血漿，細胞培養上清及其他生物學樣本
  • 檢測樣本體積：20 μL
  • 靈敏度：1.73 pg/mL
  • 檢測范圍：7.81 pg/mL - 500 pg/mL
  • 回收率：92% - 127%

在售SKU:70-EK382HS-48, 70-EK382HS-96

描述

商品名

Rat TNF-α High Sensitivity ELISA Kit (大鼠腫瘤壞死因子α高敏 ELISA試劑盒)
檢測方法

雙抗夾心法
精密度

板內變異系數：4.2% - 4.8%；板間變異系數：3.7% - 10.1%
樣本類型

血清，血漿，細胞培養上清及其他生物學樣本
檢測樣本體積

20 μL
靈敏度

1.73 pg/mL
檢測范圍

7.81 pg/mL - 500 pg/mL
回收率

92% - 127%
平均回收率

1.12
板式

96孔板，可拆
保存

試劑盒未拆開，4℃保存。已拆開，標準品-20℃保存，其它4℃保存。
運輸條件

4℃藍冰運輸
組分
  • 包被抗TNF-α單克隆抗體的96孔聚苯乙烯酶標板
  • 大鼠TNF-α凍干標準品
  • TNF-α檢測抗體
  • 標準品稀釋液
  • 辣根過氧化物酶標記的鏈霉親和素
  • 信號增強劑濃縮液
  • 信號增強劑稀釋液
  • 檢測緩沖液(10×)
  • 底物(TMB)
  • 終止液
  • 洗液(20×)
  • 封板膜

檢測原理：本試劑盒采用雙抗體夾心酶聯免疫吸附檢測技術。特異性抗大鼠TNF-α抗體預包被在高親和力的酶標板上。酶標板孔中加入標準品和待測樣本，經過孵育，樣本中存在的TNF-α與固相抗體結合。洗滌去除未結合的物質后，加入生物素化的檢測抗體孵育。洗滌去除未結合的生物素化的抗體，加入辣根過氧化物酶標記的鏈霉親和素 (Streptavidin-HRP)。洗滌后，加入信號增強劑孵育，洗滌去除未結合的物質后，再次加入Streptavidin-HRP。洗滌后，加入顯色底物TMB，避光顯色。顏色反應的深淺與樣本中TNF-α的濃度成正比。加入終止液終止反應，在450 nm波長(參考波長570 - 630 nm)測定吸光度值。

Rat TNF-α High Sensitivity ELISA Kit (大鼠腫瘤壞死因子α高敏 ELISA試劑盒) - 標準曲線
標準曲線

分子信息

概述大鼠 TNF, TNFA, TNFSF2 靶點信息

TNF 分子靶點信息概述

  • 分子名：TNF, tumor necrosis factor
  • 基因家族：Tumor necrosis factor superfamily
  • 別名：TNFSF2; DIF; TNF-alpha
  • 曾用名：TNFA
  • 全稱：TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2)

TNF 分子靶點綜述

TNF-α 為單核細胞、巨噬細胞、中性粒細胞、NK 細胞和 CD4+T 細胞產生的 17 kDa 細胞因子。它在免疫應答中具有多種調節功能，還可作為潛在的熱原。TNF-α對刺激物(感染因子或組織受損)產生應答后，在全身循環，激活中性粒細胞，改變血管內皮細胞的特性，調控其它組織的代謝活性，以及通過誘導局部凝血作用展現腫瘤殺傷活性。TNF-α 在關節組織和其它組織發生炎癥的發病機制具有重要作用。

大鼠 Rat Tnf 分子靶點信息

  • 分子名：Tnf, tumor necrosis factor
  • 別稱：
    • cachectin
    • LOC103694380
    • MGC124630
    • RATTNF
    • TNF-a
    • TNF-alpha
    • Tnfa
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor superfamily member 2
    • tumor necrosis factor superfamily, member 2
    • tumor necrosis factor-alpha
    • tumor necrosis factor-like
  • 基因序列：NCBI_Gene: 24835
  • 蛋白序列：UniProtKB: P16599

大鼠 Rat Tnf 靶點分子功能（預測）

Enables tumor necrosis factor receptor binding activity. Involved in several processes, including positive regulation of cell communication; positive regulation of macromolecule metabolic process; and positive regulation of neuron death. Located in several cellular components, including external side of plasma membrane; extracellular space; and neuronal cell body. Used to study several diseases, including cerebrovascular disease (multiple); liver disease (multiple); peptic esophagitis; perinatal necrotizing enterocolitis; and periventricular leukomalacia. Biomarker of several diseases, including brain disease (multiple); congestive heart failure (multiple); inflammatory bowel disease (multiple); lung disease (multiple); and non-alcoholic fatty liver disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); eye disease (multiple); lung disease (multiple); and skin disease (multiple). Orthologous to human TNF (tumor necrosis factor).

引用文獻統計

該產品被引用的文獻總數為：358

高敏試劑盒操作常見問題

小心！你的 ELISA 試劑盒可能是假的

小心！你的 ELISA 試劑盒可能是假的 

開學學習計劃，如何收集ELISA檢測樣本——細胞

開學學習計劃，如何收集ELISA檢測樣本——細胞 

開學學習計劃，如何選擇ELISA試劑盒

開學學習計劃，如何選擇ELISA試劑盒 

開學學習計劃，ELISA從新手到入門！

開學學習計劃，ELISA從新手到入門！ 

一文掌握ELISA實驗顯色判斷、數據分析及標曲擬合

一文掌握ELISA實驗顯色判斷、數據分析及標曲擬合 

血清OR血漿，哪個是ELISA的菜

血清OR血漿，哪個是ELISA的菜 

ELISA通關必備丨數據篇丨標準曲線不佳

ELISA通關必備丨數據篇丨標準曲線不佳 

ELISA通關必備丨操作篇丨常見問題及解決方案

ELISA通關必備丨操作篇丨常見問題及解決方案 

ELISA通關必備丨操作篇丨溶解與稀釋標準品

ELISA通關必備丨操作篇丨溶解與稀釋標準品 

ELISA通關必備丨樣本篇丨不常見樣本

ELISA通關必備丨樣本篇丨不常見樣本 

ELISA通關必備丨樣本篇丨常見樣本丨細胞

ELISA通關必備丨樣本篇丨常見樣本丨細胞 

ELISA通關必備丨如何選擇試劑盒

ELISA通關必備丨如何選擇試劑盒 

查看更多高敏試劑盒操作步驟技術文章

操作步驟

ELISA操作常見問題

小心！你的 ELISA 試劑盒可能是假的 

開學學習計劃，如何收集ELISA檢測樣本——細胞 

開學學習計劃，如何選擇ELISA試劑盒 

開學學習計劃，ELISA從新手到入門！ 

一文掌握ELISA實驗顯色判斷、數據分析及標曲擬合 

血清OR血漿，哪個是ELISA的菜 

ELISA通關必備丨數據篇丨標準曲線不佳 

ELISA通關必備丨操作篇丨常見問題及解決方案 

ELISA通關必備丨操作篇丨溶解與稀釋標準品 

ELISA通關必備丨樣本篇丨不常見樣本 

ELISA通關必備丨樣本篇丨常見樣本丨細胞 

ELISA通關必備丨如何選擇試劑盒 

ELISA通關必備丨基礎知識 

真？假？ELISA試劑盒選擇要小心 

ELISA常見類型一 | 雙抗夾心法，你要的都在這里！ 

ELISA常見類型二 | 競爭法，五分鐘搞定！ 

叮！聯科向您投遞了個ELISA實驗操作干貨包，請查收~ 

【視頻】ELISA實驗操作步驟演示視頻教程 

ELISA 組織樣本的處理—大鼠組織 

【視頻】ELISA實驗原理與常見問題分析 

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引用文獻

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