EK306

Rat IL-6 ELISA Kit檢測試劑盒（酶聯免疫吸附法）

X

¥1,600.00 – ¥10,800.00

因產品會迭代升級，具體實驗步驟請按紙質版說明書操作

  • 分子靶點：IL6, IL-6, IFNB2, HGF
  • 種屬：大鼠 (Rat)
  • 樣本類型：血清，血漿，細胞培養上清及其他生物學樣本
  • 檢測樣本體積：20 μL
  • 靈敏度：2.57 pg/mL
  • 檢測范圍：31.25 pg/mL - 2000 pg/mL
  • 回收率：92% - 104%

在售SKU:70-EK306/3-48, 70-EK306/3-96

描述

商品名

Rat IL-6 ELISA Kit (大鼠白細胞介素6 ELISA試劑盒)
檢測方法

雙抗夾心法
精密度

板內變異系數：4.2% - 7.7%；板間變異系數：6.3% - 9.1%
樣本類型

血清，血漿，細胞培養上清及其他生物學樣本
檢測樣本體積

20 μL
靈敏度

2.57 pg/mL
檢測范圍

31.25 pg/mL - 2000 pg/mL
回收率

92% - 104%
平均回收率

0.97
板式

96孔板，可拆
保存

試劑盒未拆開，4℃保存。已拆開，標準品-20℃保存，其它4℃保存。
運輸條件

4℃藍冰運輸
組分
  • 包被抗IL-6單克隆抗體的96孔聚苯乙烯酶標板
  • 大鼠IL-6凍干標準品
  • IL-6檢測抗體
  • 標準品稀釋液
  • 檢測緩沖液(10×)
  • 底物(TMB)
  • 終止液
  • 洗液(20×)
  • 封板膜

檢測原理：本試劑盒采用雙抗體夾心酶聯免疫吸附檢測技術。特異性抗大鼠IL-6抗體預包被在高親和力的酶標板上。酶標板孔中加入標準品、待測樣本和生物素化的檢測抗體，經過孵育，樣本中存在的IL-6與固相抗體和檢測抗體結合。洗滌去除未結合的物質后，加入辣根過氧化物酶標記的鏈霉親和素(Streptavidin-HRP)。洗滌后，加入顯色底物TMB，避光顯色。顏色反應的深淺與樣本中IL-6的濃度成正比。加入終止液終止反應，在450 nm波長(參考波長570 - 630 nm)測定吸光度值。

Rat IL-6 ELISA Kit (大鼠白細胞介素6 ELISA試劑盒) - 標準曲線
標準曲線

分子信息

概述大鼠 IL6, IL-6, IFNB2, HGF 靶點信息

IL6 分子靶點信息概述

  • 分子名：IL6, interleukin 6
  • 基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
  • 別名：IL-6; BSF2; HGF; HSF
  • 曾用名：IFNB2
  • 全稱：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶點綜述

白細胞介素6(IL-6)是一種同時具備促炎性和抗炎性的細胞因子。在人類中，它由IL-6基因編碼。在感染期間和受到外傷后，尤其是燒傷或其它組織損傷導致的炎癥，IL-6會由T細胞和巨噬細胞分泌，促進免疫應答。研究表明，小鼠抵抗肺炎鏈球菌感染時需要IL-6的參與，說明IL-6在抵抗感染中發揮作用。IL-6在很多疾病如糖尿病、動脈粥樣硬化、抑郁癥、阿爾茨海默氏病、系統性紅斑狼瘡、多發性骨髓瘤、前列腺癌、白塞氏病和類風濕性關節炎等中可促進炎性和自身免疫疾病進程。晚期/轉移性癌癥患者的血液中具有較高水平的IL-6。

大鼠 Rat Il6 分子靶點信息

  • 分子名：Il6, interleukin 6
  • 別稱：
    • Ifnb2
    • IL-6
    • ILg6
    • Interleukin 6 (interferon beta 2)
    • Interleukin 6 (interferon, beta 2)
    • interleukin-6
  • 基因序列：NCBI_Gene: 24498
  • 蛋白序列：UniProtKB: P20607

大鼠 Rat Il6 靶點分子功能（預測）

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including cellular response to lipid; positive regulation of cell communication; and response to peptide hormone. Located in cytoplasm and extracellular space. Used to study several diseases, including acute necrotizing pancreatitis; disseminated intravascular coagulation; fatty liver disease; hypertension (multiple); and impotence. Biomarker of several diseases, including acute necrotizing pancreatitis; artery disease (multiple); auditory system disease (multiple); iron deficiency anemia; and lung disease (multiple). Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

引用文獻統計

該產品被引用的文獻總數為：384

ELISA試劑盒操作常見問題

小心！你的 ELISA 試劑盒可能是假的

小心！你的 ELISA 試劑盒可能是假的 

開學學習計劃，如何收集ELISA檢測樣本——細胞

開學學習計劃，如何收集ELISA檢測樣本——細胞 

開學學習計劃，如何選擇ELISA試劑盒

開學學習計劃，如何選擇ELISA試劑盒 

開學學習計劃，ELISA從新手到入門！

開學學習計劃，ELISA從新手到入門！ 

一文掌握ELISA實驗顯色判斷、數據分析及標曲擬合

一文掌握ELISA實驗顯色判斷、數據分析及標曲擬合 

血清OR血漿，哪個是ELISA的菜

血清OR血漿，哪個是ELISA的菜 

ELISA通關必備丨數據篇丨標準曲線不佳

ELISA通關必備丨數據篇丨標準曲線不佳 

ELISA通關必備丨操作篇丨常見問題及解決方案

ELISA通關必備丨操作篇丨常見問題及解決方案 

ELISA通關必備丨操作篇丨溶解與稀釋標準品

ELISA通關必備丨操作篇丨溶解與稀釋標準品 

ELISA通關必備丨樣本篇丨不常見樣本

ELISA通關必備丨樣本篇丨不常見樣本 

ELISA通關必備丨樣本篇丨常見樣本丨細胞

ELISA通關必備丨樣本篇丨常見樣本丨細胞 

ELISA通關必備丨如何選擇試劑盒

ELISA通關必備丨如何選擇試劑盒 

查看更多ELISA試劑盒操作步驟技術文章

操作步驟

ELISA操作常見問題

小心！你的 ELISA 試劑盒可能是假的 

開學學習計劃，如何收集ELISA檢測樣本——細胞 

開學學習計劃，如何選擇ELISA試劑盒 

開學學習計劃，ELISA從新手到入門！ 

一文掌握ELISA實驗顯色判斷、數據分析及標曲擬合 

血清OR血漿，哪個是ELISA的菜 

ELISA通關必備丨數據篇丨標準曲線不佳 

ELISA通關必備丨操作篇丨常見問題及解決方案 

ELISA通關必備丨操作篇丨溶解與稀釋標準品 

ELISA通關必備丨樣本篇丨不常見樣本 

ELISA通關必備丨樣本篇丨常見樣本丨細胞 

ELISA通關必備丨如何選擇試劑盒 

ELISA通關必備丨基礎知識 

真？假？ELISA試劑盒選擇要小心 

ELISA常見類型一 | 雙抗夾心法，你要的都在這里！ 

ELISA常見類型二 | 競爭法，五分鐘搞定！ 

叮！聯科向您投遞了個ELISA實驗操作干貨包，請查收~ 

【視頻】ELISA實驗操作步驟演示視頻教程 

ELISA 組織樣本的處理—大鼠組織 

【視頻】ELISA實驗原理與常見問題分析 

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引用文獻

文章目錄[隱藏]


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  26. MicroRNA-342-3p loaded by human umbilical cord mesenchymal stem cells-derived exosomes attenuates deep vein thrombosis by downregulating EDNRA 

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  31. Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway 

  32. The dynamic changes in autophagy activity and its role in lung injury after deep hypothermic circulatory arrest 

  33. Gut derived-endotoxin contributes to inflammation in severe ischemic acute kidney injury 

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  38. Telmisartan ameliorates LPS-induced pneumonia in rats through regulation of the PPARγ/NF-κB pathway 

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