EK206

Mouse IL-6 ELISA Kit檢測試劑盒（酶聯免疫吸附法）

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¥1,600.00 – ¥10,800.00

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  • 分子靶點：IL6, IL-6, IFNB2, HGF
  • 種屬：小鼠 (Mouse)
  • 樣本類型：血清，血漿，細胞培養上清及其他生物學樣本
  • 檢測樣本體積：10 μL
  • 靈敏度：0.56 pg/mL
  • 檢測范圍：7.81 pg/mL - 500 pg/mL
  • 回收率：89% - 112%

在售SKU:70-EK206/3-48, 70-EK206/3-96

描述

商品名

Mouse IL-6 ELISA Kit (小鼠白細胞介素6 ELISA試劑盒)
檢測方法

雙抗夾心法
精密度

板內變異系數：2.3% - 3.0%；板間變異系數：2.1% - 5.3%
樣本類型

血清，血漿，細胞培養上清及其他生物學樣本
檢測樣本體積

10 μL
靈敏度

0.56 pg/mL
檢測范圍

7.81 pg/mL - 500 pg/mL
回收率

89% - 112%
平均回收率

1.02
板式

96孔板，可拆
保存

試劑盒未拆開，4℃保存。已拆開，標準品-20℃保存，其它4℃保存。
運輸條件

4℃藍冰運輸
組分
  • 包被抗IL-6單克隆抗體的96孔聚苯乙烯酶標板
  • 小鼠IL-6凍干標準品
  • IL-6檢測抗體
  • 標準品稀釋液
  • 檢測緩沖液(10×)
  • 底物(TMB)
  • 終止液
  • 洗液(20×)
  • 封板膜

檢測原理：本試劑盒采用雙抗體夾心酶聯免疫吸附檢測技術。特異性抗小鼠IL-6抗體預包被在高親和力的酶標板上。酶標板孔中加入標準品、待測樣本和生物素化的檢測抗體，經過孵育，樣本中存在的IL-6與固相抗體和檢測抗體結合。洗滌去除未結合的物質后，加入辣根過氧化物酶標記的鏈霉親和素(Streptavidin-HRP)。洗滌后，加入顯色底物TMB，避光顯色。顏色反應的深淺與樣本中IL-6的濃度成正比。加入終止液終止反應，在450 nm波長(參考波長570 - 630 nm)測定吸光度值。

Mouse IL-6 ELISA Kit (小鼠白細胞介素6 ELISA試劑盒) - 標準曲線
標準曲線

分子信息

概述小鼠 IL6, IL-6, IFNB2, HGF 靶點信息

IL6 分子靶點信息概述

  • 分子名：IL6, interleukin 6
  • 基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
  • 別名：IL-6; BSF2; HGF; HSF
  • 曾用名：IFNB2
  • 全稱：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶點綜述

白細胞介素6(IL-6)是一種同時具備促炎性和抗炎性的細胞因子。在人類中，它由IL-6基因編碼。在感染期間和受到外傷后，尤其是燒傷或其它組織損傷導致的炎癥，IL-6會由T細胞和巨噬細胞分泌，促進免疫應答。研究表明，小鼠抵抗肺炎鏈球菌感染時需要IL-6的參與，說明IL-6在抵抗感染中發揮作用。IL-6在很多疾病如糖尿病、動脈粥樣硬化、抑郁癥、阿爾茨海默氏病、系統性紅斑狼瘡、多發性骨髓瘤、前列腺癌、白塞氏病和類風濕性關節炎等中可促進炎性和自身免疫疾病進程。晚期/轉移性癌癥患者的血液中具有較高水平的IL-6。

小鼠 Mouse Il6 分子靶點信息

小鼠 Mouse Il6 靶點分子功能（預測）

Enables cytokine activity and interleukin-6 receptor binding activity. Involved in several processes, including animal organ development; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within with a positive effect on gene expression. Acts upstream of or within several processes, including cellular response to cytokine stimulus; regulation of gene expression; and salivary gland morphogenesis. Located in external side of plasma membrane and extracellular space. Part of interleukin-6 receptor complex. Is expressed in several structures, including adrenal gland; genitourinary system; gut; liver; and thymus. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); autoimmune disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Orthologous to human IL6 (interleukin 6).

引用文獻統計

該產品被引用的文獻總數為：1296

ELISA試劑盒操作常見問題

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小心！你的 ELISA 試劑盒可能是假的 

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開學學習計劃，如何選擇ELISA試劑盒

開學學習計劃，如何選擇ELISA試劑盒 

開學學習計劃，ELISA從新手到入門！

開學學習計劃，ELISA從新手到入門！ 

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ELISA通關必備丨操作篇丨常見問題及解決方案

ELISA通關必備丨操作篇丨常見問題及解決方案 

ELISA通關必備丨操作篇丨溶解與稀釋標準品

ELISA通關必備丨操作篇丨溶解與稀釋標準品 

ELISA通關必備丨樣本篇丨不常見樣本

ELISA通關必備丨樣本篇丨不常見樣本 

ELISA通關必備丨樣本篇丨常見樣本丨細胞

ELISA通關必備丨樣本篇丨常見樣本丨細胞 

ELISA通關必備丨如何選擇試劑盒

ELISA通關必備丨如何選擇試劑盒 

查看更多ELISA試劑盒操作步驟技術文章

操作步驟

ELISA操作常見問題

小心！你的 ELISA 試劑盒可能是假的 

開學學習計劃，如何收集ELISA檢測樣本——細胞 

開學學習計劃，如何選擇ELISA試劑盒 

開學學習計劃，ELISA從新手到入門！ 

一文掌握ELISA實驗顯色判斷、數據分析及標曲擬合 

血清OR血漿，哪個是ELISA的菜 

ELISA通關必備丨數據篇丨標準曲線不佳 

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ELISA通關必備丨操作篇丨溶解與稀釋標準品 

ELISA通關必備丨樣本篇丨不常見樣本 

ELISA通關必備丨樣本篇丨常見樣本丨細胞 

ELISA通關必備丨如何選擇試劑盒 

ELISA通關必備丨基礎知識 

真？假？ELISA試劑盒選擇要小心 

ELISA常見類型一 | 雙抗夾心法，你要的都在這里！ 

ELISA常見類型二 | 競爭法，五分鐘搞定！ 

叮！聯科向您投遞了個ELISA實驗操作干貨包，請查收~ 

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引用文獻

文章目錄[隱藏]


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  5. P21 activated kinase-1 (PAK1) in macrophages is required for promotion of Th17 cell response during helminth infection 

  6. The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis 

  7. 5-Methoxyflavone alleviates LPS-mediated lung injury by promoting Nrf2-mediated the suppression of NOX4/TLR4 axis in bronchial epithelial cells?and M1 polarization in macrophages 

  8. S-allylmercaptocysteine inhibits mucin overexpression and inflammation via MAPKs and PI3K-Akt signaling pathways in acute respiratory distress syndrome 

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  10. Anti-inflammatory activity of Anchusa italica Retz. in LPS-stimulated RAW264.7?cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways 

  11. Propionate alleviated post-infarction cardiac dysfunction by macrophage polarization in a rat model 

  12. Berberine alleviates myocardial ischemia–reperfusion injury by inhibiting inflammatory response and oxidative stress: the key function of miR-26b-5p-mediated PTGS2/MAPK signal transduction 

  13. Serum amyloid A1 exacerbates hepatic steatosis via TLR4-mediated NF-κB signaling pathway 

  14. Fullerenols boosting the therapeutic effect of anti-CD47 antibody to trigger robust anti-tumor immunity by inducing calreticulin exposure 

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  16. Phloretin inhibited the pathogenicity and virulence factors against Candida albicans 

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  19. S-allylmercapto-N-acetylcysteine ameliorates pulmonary fibrosis in mice via Nrf2 pathway activation and NF-κB, TGF-β1/Smad2/3 pathway suppression 

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  30. The Regulatory Effects of Licochalcone A on the Intestinal Epithelium and Gut Microbiota in Murine Colitis 

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