EK106

Human IL-6 ELISA Kit檢測試劑盒（酶聯免疫吸附法）

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¥1,600.00 – ¥10,800.00

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  • 分子靶點：IL6, IL-6, IFNB2, HGF
  • 種屬：人 (Human)
  • 樣本類型：血清，血漿，細胞培養上清及其他生物學樣本
  • 檢測樣本體積：50 μL
  • 靈敏度：0.37 pg/mL
  • 檢測范圍：1.56 pg/mL - 100 pg/mL
  • 回收率：99% - 115%

在售SKU:70-EK106/2-48, 70-EK106/2-96, 人白細胞介素6, 白細胞介素6, 白細胞介素, 人, 白介素, 6

描述

商品名

Human IL-6 ELISA Kit (人白介素6 ELISA試劑盒)
檢測方法

雙抗夾心法
精密度

板內變異系數：2.5%-5.0%；板間變異系數：1.9% - 4.6%
樣本類型

血清，血漿，細胞培養上清及其他生物學樣本
檢測樣本體積

50 μL
靈敏度

0.37 pg/mL
檢測范圍

1.56 pg/mL - 100 pg/mL
回收率

99% - 115%
平均回收率

1.06
板式

96孔板，可拆
保存

試劑盒未拆開，4℃保存。已拆開，標準品-20℃保存，其它4℃保存。
運輸條件

4℃藍冰運輸
組分
  • 包被抗IL-6單克隆抗體的96孔聚苯乙烯酶標板
  • 人IL-6凍干標準品
  • IL-6檢測抗體
  • 標準品稀釋液
  • 檢測緩沖液(10×)
  • 底物(TMB)
  • 終止液
  • 洗液(20×)
  • 封板膜

檢測原理：本試劑盒采用雙抗體夾心酶聯免疫吸附檢測技術。特異性抗人IL-6抗體預包被在高親和力的酶標板上。酶標板孔中加入標準品、待測樣本和生物素化的檢測抗體，經過孵育，樣本中存在的IL-6與固相抗體和檢測抗體結合。洗滌去除未結合的物質后，加入辣根過氧化物酶標記的鏈霉親和素(Streptavidin-HRP)。洗滌后，加入顯色底物TMB，避光顯色。顏色反應的深淺與樣本中IL-6的濃度成正比。加入終止液終止反應，在450 nm波長(參考波長570 - 630 nm)測定吸光度值。

Human IL-6 ELISA Kit (人白介素6 ELISA試劑盒) - 標準曲線
標準曲線

分子信息

概述人 IL6, IL-6, IFNB2, HGF 靶點信息

IL6 分子靶點信息概述

  • 分子名：IL6, interleukin 6
  • 基因家族：Interferons; Interleukins; Interleukin 6 type cytokine family
  • 別名：IL-6; BSF2; HGF; HSF
  • 曾用名：IFNB2
  • 全稱：interferon, beta 2; interleukin 6 (interferon, beta 2)

IL6 分子靶點綜述

白細胞介素6(IL-6)是一種同時具備促炎性和抗炎性的細胞因子。在人類中，它由IL-6基因編碼。在感染期間和受到外傷后，尤其是燒傷或其它組織損傷導致的炎癥，IL-6會由T細胞和巨噬細胞分泌，促進免疫應答。研究表明，小鼠抵抗肺炎鏈球菌感染時需要IL-6的參與，說明IL-6在抵抗感染中發揮作用。IL-6在很多疾病如糖尿病、動脈粥樣硬化、抑郁癥、阿爾茨海默氏病、系統性紅斑狼瘡、多發性骨髓瘤、前列腺癌、白塞氏病和類風濕性關節炎等中可促進炎性和自身免疫疾病進程。晚期/轉移性癌癥患者的血液中具有較高水平的IL-6。

人 Human IL6 分子靶點信息

  • 分子名：IL6, interleukin 6
  • 別稱：
    • B cell stimulatory factor-2
    • B-cell differentiation factor
    • B-cell stimulatory factor 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • HGF
    • HSF
    • hybridoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL-6
    • interferon beta-2
    • interferon, beta 2
    • interleukin BSF-2
    • interleukin-6
  • 基因序列：NCBI_Gene: 3569
  • 蛋白序列：UniProtKB: P05231

人 Human IL6靶點分子功能（預測）

Enables cytokine activity and growth factor activity. Contributes to interleukin-6 receptor binding activity. Involved in several processes, including positive regulation of cell population proliferation; positive regulation of macromolecule metabolic process; and regulation of peptide hormone secretion. Acts upstream of or within several processes, including cellular response to lipopolysaccharide; positive regulation of leukocyte adhesion to vascular endothelial cell; and positive regulation of macromolecule metabolic process. Located in extracellular space. Part of interleukin-6 receptor complex. Implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and periodontal disease (multiple). Biomarker of several diseases, including autoimmune disease (multiple); eye disease (multiple); gastrointestinal system cancer (multiple); glucose metabolism disease (multiple); and lung disease (multiple).

引用文獻統計

該產品被引用的文獻總數為：422

ELISA試劑盒操作常見問題

小心！你的 ELISA 試劑盒可能是假的

小心！你的 ELISA 試劑盒可能是假的 

開學學習計劃，如何收集ELISA檢測樣本——細胞

開學學習計劃，如何收集ELISA檢測樣本——細胞 

開學學習計劃，如何選擇ELISA試劑盒

開學學習計劃，如何選擇ELISA試劑盒 

開學學習計劃，ELISA從新手到入門！

開學學習計劃，ELISA從新手到入門！ 

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ELISA通關必備丨操作篇丨常見問題及解決方案

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ELISA通關必備丨操作篇丨溶解與稀釋標準品

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ELISA通關必備丨樣本篇丨不常見樣本

ELISA通關必備丨樣本篇丨不常見樣本 

ELISA通關必備丨樣本篇丨常見樣本丨細胞

ELISA通關必備丨樣本篇丨常見樣本丨細胞 

ELISA通關必備丨如何選擇試劑盒

ELISA通關必備丨如何選擇試劑盒 

查看更多ELISA試劑盒操作步驟技術文章

操作步驟

ELISA操作常見問題

小心！你的 ELISA 試劑盒可能是假的 

開學學習計劃，如何收集ELISA檢測樣本——細胞 

開學學習計劃，如何選擇ELISA試劑盒 

開學學習計劃，ELISA從新手到入門！ 

一文掌握ELISA實驗顯色判斷、數據分析及標曲擬合 

血清OR血漿，哪個是ELISA的菜 

ELISA通關必備丨數據篇丨標準曲線不佳 

ELISA通關必備丨操作篇丨常見問題及解決方案 

ELISA通關必備丨操作篇丨溶解與稀釋標準品 

ELISA通關必備丨樣本篇丨不常見樣本 

ELISA通關必備丨樣本篇丨常見樣本丨細胞 

ELISA通關必備丨如何選擇試劑盒 

ELISA通關必備丨基礎知識 

真？假？ELISA試劑盒選擇要小心 

ELISA常見類型一 | 雙抗夾心法，你要的都在這里！ 

ELISA常見類型二 | 競爭法，五分鐘搞定！ 

叮！聯科向您投遞了個ELISA實驗操作干貨包，請查收~ 

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引用文獻

文章目錄[隱藏]

  1. RORγt inhibitor SR1001 alleviates acute pancreatitis by suppressing pancreatic IL-17-producing Th17 and γδ-T cells in mice with ceruletide-induced pancreatitis 

  2. Tumor associated neutrophils promote the metastasis of pancreatic ductal adenocarcinoma 

  3. Accumulation of T?helper 22 cells, interleukin?22 and myeloid?derived suppressor cells promotes gastric cancer progression in elderly patients 

  4. The role of alcohol dehydrogenase 1C in regulating inflammatory responses in ulcerative colitis 

  5. Cathelicidin antimicrobial peptides suppress EV71 infection via regulating antiviral response and inhibiting viral binding 

  6. Suppression of NOD-like receptor protein 3 inflammasome activation and macrophage M1 polarization by hederagenin contributes to attenuation of sepsis-induced acute lung injury in rats 

  7. Construction of PD1/CD28 chimeric-switch receptor enhances anti-tumor ability of c-Met CAR-T in gastric cancer 

  8. Involvement of impaired CD8+ mucosal-associated invariant T cells and myeloid-derived suppressor cells in polycystic ovary syndrome 

  9. BRAFV600E mutant colorectal cancer cells mediate local immunosuppressive microenvironment through exosomal long noncoding RNAs 

  10. Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway 

  11. Timosaponin B-II alleviates osteoarthritis-related inflammation and extracellular matrix degradation through inhibition of mitogen-activated protein kinases and nuclear factor-κB pathways in vitro 

  12. Consumption of Saturated Fatty Acids-Rich Lard Benefits Recovery of Experimental Arthritis by Activating PPAR-γ 

  13. Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling 

  14. Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling 

  15. Smoking increases oral mucosa susceptibility to Candida albicans infection via the Nrf2 pathway: In vitro and animal studies 

  16. Chronic exposure to microcystin-leucine-arginine induces epithelial hyperplasia and inflammation in the mouse bladder 

  17. Oral Core–Shell Nanoparticles Embedded in Hydrogel Microspheres for the Efficient Site-Specific Delivery of Magnolol and Enhanced Antiulcerative Colitis Therapy 

  18. Biomimetic Macrophage Membrane and Lipidated Peptide Hybrid Nanovesicles for Atherosclerosis Therapy 

  19. miR-146a is directly regulated by STAT3 in human hepatocellular carcinoma cells and involved in anti-tumor immune suppression 

  20. Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling 

  21. Catalpol ameliorates psoriasis-like phenotypes via SIRT1 mediated suppression of NF-κB and MAPKs signaling pathways 

  22. Paclitaxel alleviated sepsis-induced acute lung injury by activating MUC1 and suppressing TLR-4/NF-κB pathway 

  23. Ginsenoside Rg3 suppresses the NLRP3 inflammasome activation through inhibition of its assembly 

  24. MiR-128-3p inhibits vascular smooth muscle cell proliferation and migration by repressing FOXO4/MMP9 signaling pathway 

  25. Brilliant blue G attenuates neuro?inflammation via regulating MAPKs and NF?κB signaling pathways in lipopolysaccharide?induced BV2 microglia cells 

  26. Cimifugin ameliorates imiquimod-induced psoriasis by inhibiting oxidative stress and inflammation via NF-κB/MAPK pathway 

  27. MiR-29c protects against inflammation and apoptosis in Parkinson's disease model in vivo and in vitro by targeting SP1 

  28. P38 MAPK, NF-κB, and JAK-STAT3 Signaling Pathways Involved in Capecitabine-Induced Hand-Foot Syndrome via Interleukin 6 or Interleukin 8 Abnormal Expression 

  29. Downregulating lncRNA PRNCR1 ameliorates LPS-induced pulmonary vascular endothelial cell injury by modulating miR-330-5p/TLR4 axis 

  30. Enhanced antitumor effect of combination of annexin A1 knockdown and bortezomib treatment in multiple myeloma in?vitro and in?vivo 

  31. Fluorous-Tagged Peptide Nanoparticles Ameliorate Acute Lung Injury via Lysosomal Stabilization and Inflammation Inhibition in Pulmonary Macrophages 

  32. Mycobacterial PPE13 activates inflammasome by interacting with the NATCH and LRR domains of NLRP3 

  33. p-Hydroxylcinnamaldehyde induces the differentiation of oesophageal carcinoma cells via the cAMP-RhoA-MAPK signalling pathway 

  34. IL (Interleukin)-6 Contributes to Deep Vein Thrombosis and Is Negatively Regulated by miR-338-5p 

  35. Thioredoxin relieves lipopolysaccharide?induced acute kidney injury in mice by reducing inflammation, oxidative stress and apoptosis 

  36. Platelet-derived growth factor B attenuates lethal sepsis through inhibition of inflammatory responses 

  37. RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein 

  38. Polysaccharides from Dendrobium officinale ameliorate colitis-induced lung injury via inhibiting inflammation and oxidative stress 

  39. Polysaccharides from Platycodonis Radix ameliorated respiratory syncytial virus-induced epithelial cell apoptosis and inflammation through activation of miR-181a-mediated Hippo and SIRT1 pathways 

  40. Short-term efficacy, safety and survival of laparoscopic radical resection for colorectal cancer patients with bowel obstruction 

  41. β-Adrenoceptor regulates contraction and inflammatory cytokine?expression of human bladder smooth muscle cells via autophagy under pathological hydrostatic pressure 

  42. Single Administration of Ultra-Low-Dose Lipopolysaccharide in Rat Early Pregnancy Induces TLR4 Activation in the Placenta Contributing to Preeclampsia 

  43. Galectin-3 exacerbates ox-LDL-mediated endothelial injury by inducing inflammation via integrin β1-RhoA-JNK signaling activation 

  44. Piperlongumine induces apoptosis and reduces bortezomib resistance by inhibiting STAT3 in multiple myeloma cells 

  45. Puerarin Prevents Acute Liver Injury via Inhibiting Inflammatory Responses and ZEB2 Expression 

  46. Effects of Transcutaneous Electrical Acupoint Stimulation on Systemic Inflammatory Response Syndrome of Patients after Percutaneous Nephrolithotomy: A Randomized Controlled Trial 

  47. Cytokine nanosponges suppressing overactive macrophages and dampening systematic cytokine storm for the treatment of hemophagocytic lymphohistiocytosis 

  48. Chlorogenic acid improves intestinal barrier function by downregulating CD14 to inhibit the NF-κB signaling pathway 

  49. Knockdown of lncRNA NORAD inhibits the proliferation, inflammation and fibrosis of human mesangial cells under high?glucose conditions by regulating the miR?485/NRF1 axis 

  50. Long non?coding RNA?HOTAIR knockdown alleviates lipopolysaccharide?induced acute respiratory distress syndrome and the associated inflammatory response by modulating the microRNA?30a?5p/PDE7A axis 

  51. Inflammatory Cytokines Changed in Patients With Depression Before and After Repetitive Transcranial Magnetic Stimulation Treatment 

  52. Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway 

  53. Liraglutide regulates bone destruction and exhibits anti-inflammatory effects in periodontitis in vitro and in vivo 

  54. Gypenosides Protect Orbital Fibroblasts in Graves Ophthalmopathy via Anti-Inflammation and Anti-Fibrosis Effects 

  55. Effects of the Chinese herbal medicine Hong Huang decoction, on myocardial injury in breast cancer patients who underwent anthracycline-based chemotherapy 

  56. Inhibition of microRNA-92a ameliorates lipopolysaccharide-induced endothelial barrier dysfunction by targeting ITGA5 through the PI3K/Akt signaling pathway in human pulmonary microvascular endothelial cells 

  57. Promotion of angiogenesis in vitro by Astragalus polysaccharide via activation of TLR4 signaling pathway 

  58. Protective mechanisms of Zanthoxylum bungeanum essential oil on DSS-induced ulcerative colitis in mice based on a colonic mucosal transcriptomic approach 

  59. Atorvastatin inhibits renal inflammatory response induced by calcium oxalate crystals via inhibiting the activation of TLR4/NF-κB and NLRP3 inflammasome 

  60. Development, characterisation, and in vitro anti-tumor effect of self-microemulsifying drug delivery system containing polyphyllin I 

  61. Nucleotide-binding oligomerization domain (NOD) plays an important role in neonatal infection 

  62. Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling 

  63. Vitamin D deficiency is associated with thyroid autoimmunity: results from an epidemiological survey in Tianjin, China 

  64. Overexpression of Forkhead box C1 attenuates oxidative stress, inflammation and apoptosis in chronic obstructive pulmonary disease 

  65. Insulin Exacerbates Inflammation in Fibroblast-Like Synoviocytes 

  66. Effects of β2/aβ2 on oxLDL-induced CD36 activation in THP-1 macrophages 

  67. Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells 

  68. MiR-146a-5p Mimic Inhibits NLRP3 Inflammasome Downstream Inflammatory Factors and CLIC4 in Neonatal Necrotizing Enterocolitis 

  69. Ex vivo lung perfusion with perfusate purification for human donor lungs following prolonged cold storage 

  70. A cross-sectional study reveals a chronic low-grade inflammation in achalasia 

  71. hnRNPA2/B1 Ameliorates LPS-Induced Endothelial Injury through NF-κB Pathway and VE-Cadherin/β-Catenin Signaling Modulation In Vitro 

  72. Progesterone Suppresses Neisseria gonorrhoeae-Induced Inflammation Through Inhibition of NLRP3 Inflammasome Pathway in THP-1 Cells and Murine Models 

  73. MicroRNA-92a serves as a risk factor in sepsis-induced ARDS and regulates apoptosis and cell migration in lipopolysaccharide-induced HPMEC and A549 cell injury 

  74. Evaluation of in vitro anti-acne activities of Ocimum basilicum L. water extract 

  75. AEG-1 silencing attenuates M2-polarization of glioma-associated microglia/macrophages and sensitizes glioma cells to temozolomide 

  76. Inflammatory cytokine?induced expression of MASTL is involved in hepatocarcinogenesis by regulating cell cycle progression 

  77. MicroRNA-181b stimulates inflammation via the nuclear factor-κB signaling pathway in vitro 

  78. α-Synuclein induces Th17 differentiation and impairs the function and stability of Tregs by promoting RORC transcription in Parkinson's disease 

  79. OxLDL/β2GPI/anti?β2GPI Ab complex induces inflammatory activation via the TLR4/NF?κB pathway in HUVECs 

  80. Polygonatum sibiricum polysaccharide alleviates inflammatory cytokines and promotes glucose uptake in high?glucose? and high?insulin?induced 3T3?L1 adipocytes by promoting Nrf2 expression 

  81. LncRNA XIST knockdown ameliorates oxidative low-density lipoprotein-induced endothelial cells injury by targeting miR-204-5p/TLR4 

  82. Progesterone-induced blocking factor-mediated Th1/Th2 balance correlates with fetal arrest in women who underwent in vitro fertilization and embryo transfer 

  83. Elevated Peripheral Frequencies of Th22 Cells: A Novel Potent Participant in Obesity and Type 2 Diabetes 

  84. MicroRNA 27b-3p Modulates SYK in Pediatric Asthma Induced by Dust Mites 

  85. Cytokine-induced killer cells efficiently kill stem-like cancer cells of nasopharyngeal carcinoma via the NKG2D-ligands recognition 

  86. Treatment Efficacy of Chuang Ling Ye, a Traditional Chinese Herbal Medicine Compound, on Idiopathic Granulomatous Mastitis: A Randomized Controlled Trial 

  87. Simultaneous blocking of CD47 and PD-L1 increases innate and adaptive cancer immune responses and cytokine release 

  88. A novel long-term intravenous combined with local treatment with human amnion-derived mesenchymal stem cells for a multidisciplinary rescued uremic calciphylaxis patient and the underlying mechanism 

  89. Inverse relationship between toxic shock syndrome toxin-1 antibodies and interferon-γ and interleukin-6 in peripheral blood mononuclear cells from patients with pediatric tonsillitis caused by Staphylococcus aureus 

  90. Nanobody-based CAR T cells targeting intracellular tumor antigens 

  91. Alteration in the Cytokine Secretion of Bone Marrow Stromal Cells from Patients with Chronic Myelomonocytic Leukemia Contribute to Impaired Hematopoietic Supportive Activity 

  92. CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF-κB Pathway 

  93. LncNSPL facilitates influenza A viral immune escape by restricting TRIM25-mediated K63-linked RIG-I ubiquitination 

  94. Hypaphorine exerts anti-inflammatory effects in sepsis induced acute lung injury via modulating DUSP1/p38/JNK pathway 

  95. Wnt/β-catenin signaling mediates the abnormal osteogenic and adipogenic capabilities of bone marrow mesenchymal stem cells from chronic graft-versus-host disease patients 

  96. Tonsillar Microbiome-Derived Lantibiotics Induce Structural Changes of IL-6 and IL-21 Receptors and Modulate Host Immunity 

  97. Expression profile of PU.1 in CD4+T cells from patients with systemic lupus erythematosus 

  98. Changes in peripheral blood cytokines in patients with severe fever with thrombocytopenia syndrome 

  99. Downregulation of TIGIT Expression in FOXP3+Regulatory T Cells in Acute Coronary Syndrome 

  100. The relationship between dyslipidemia and inflammation among adults in east coast China: A cross-sectional study 

  101. Sprouty2 regulates proliferation and survival of multiple myeloma by?inhibiting activation of the ERK1/2 pathway in?vitro and in?vivo 

  102. Chronic Microcystin-LR-Induced α-Synuclein Promotes Neuroinflammation Through Activation of the NLRP3 Inflammasome in Microglia 

  103. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFκB signaling-mediated autophagy 

  104. Metabolic and Network Pharmacological Analyses of the Therapeutic Effect of Grona styracifolia on Calcium Oxalate-Induced Renal Injury 

  105. Quantum dots bind nanosheet to promote nanomaterial stability and resist endotoxin-induced fibrosis and PM2.5-induced pneumonia 

  106. IL-33 receptor expression on myeloid and plasmacytoid dendritic cells after allergen challenge in patients with allergic rhinitis 

  107. Berberine inhibits dendritic cells differentiation in DSS-induced colitis by promoting Bacteroides fragilis 

  108. Comparison and relationship of thyroid hormones, IL-6, IL-10 and albumin as mortality predictors in case-mix critically ill patients 

  109. Glucose Metabolism Reprogramming of Regulatory T Cells in Concanavalin A-Induced Hepatitis 

  110. Potential biomarkers for diagnosis and assessment of disease activity in systemic lupus erythematosus 

  111. Comprehensive Analysis of NAFLD and the Therapeutic Target Identified 

  112. Diallyl trisulfide inhibits naphthalene-induced oxidative injury and the production of inflammatory responses in A549 cells and mice 

  113. Different effects of lipoteichoic acid from C. butyricum and S. aureus on inflammatory responses of HT-29 cells 

  114. TLR2 expression doesn’t change in ox-LDL mediated inflammation in Human umbilical vein endothelial cells under high glucose culture 

  115. Participation of Increased Circulating MAIT Cells in Lung Cancer: a Pilot Study 

  116. The effect of liraglutide on the proliferation, migration, and osteogenic differentiation of human periodontal ligament cells 

  117. Interleukin 1β and tumor necrosis factor α promote hFOB1.19 cell viability via activating AP1 

  118. Serum NOX2 as a new biomarker candidate for HBV-related disorders 

  119. Berberine relieves insulin resistance via the cholinergic anti-inflammatory pathway in HepG2 cells 

  120. NANOG Promotes Cell Proliferation, Invasion, and Stemness via IL-6/STAT3 Signaling in Esophageal Squamous Carcinoma 

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