CCS012

Cell Cycle Staining Kit 細胞周期檢測試劑盒

X

¥390.00

在售SKU:70-CCS012, 細胞周期, 周期試劑盒, 細胞周期試劑盒, 周期, 細胞周期檢測, 試劑盒


描述

文章目錄[隱藏]

產品簡介

碘化丙啶(Propidium iodide, PI)可插入堿基之間而與DNA結合,其對堿基幾乎沒有序列偏好,每4 - 5個堿基對可結合一個染料分子。PI也能與RNA結合,需要用核酸酶處理來區分RNA和DNA染色。一旦染料與核酸結合,其熒光會增強20 - 30倍。雖然其摩爾吸收率(吸光系數)相對較低,PI仍然具有較大的stokes位移,從而在濾光片選擇恰當的情況下,仍能同時檢測細胞核DNA和熒光標記的抗體。PI可用于熒光顯微鏡、激光共聚焦、流式細胞儀和熒光計。

PI為膜不通透性,被活細胞排斥,可用于鑒定死細胞和多色熒光復染。下面提供的操作步驟與很多細胞標記技術兼容,如直接或間接抗體檢測技術、mRNA原位雜交、特異性的細胞結構染料等。在組織染色中可能要作修改。

本試劑盒包含50 ml DNA染色液和500 μl破膜劑,足夠用于50次檢測。

產品特點

  • 即用型,操作簡單
  • 活細胞/固定細胞檢測
  • 常備現貨

引用文獻統計

該產品被引用的文獻總數為:1190

周期試劑盒操作常見問題

查看更多周期試劑盒操作步驟技術文章

引用文獻

文章目錄[隱藏]


  1. Long non?coding RNA 01614 hyperactivates WNT/β?catenin signaling to promote pancreatic cancer progression by suppressing GSK?3β 

  2. MicroRNA bta-miR-365-3p inhibits proliferation but promotes differentiation of primary bovine myoblasts by targeting the activin A receptor type I 

  3. A cold-water polysaccharide-protein complex from Grifola frondosa exhibited antiproliferative activity via mitochondrial apoptotic and Fas/FasL pathways in HepG2 cells 

  4. Novel Affibody Molecules Targeting the HPV16 E6 Oncoprotein Inhibited the Proliferation of Cervical Cancer Cells 

  5. PLCA8 suppresses breast cancer apoptosis by activating the PI3k/AKT/NF-κB pathway 

  6. Overexpression of COPS3 promotes clear cell renal cell carcinoma progression via regulation of Phospho-AKT(Thr308), Cyclin D1 and Caspase-3 

  7. 25-HC decreases the sensitivity of human gastric cancer cells to 5-fluorouracil and promotes cells invasion via the TLR2/NF-κB signaling pathway 

  8. The role of miR-431-5p in regulating pulmonary surfactant expression in vitro 

  9. ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/β-Catenin Signaling 

  10. NUP210 and MicroRNA-22 Modulate Fas to Elicit HeLa Cell Cycle Arrest 

  11. CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells 

  12. Hsa_circRNA_101036 acts as tumor-suppressor in oral squamous cell carcinoma cells via inducing endoplasmic reticulum stress 

  13. Ureaplasma spp. lipid-associated membrane proteins induce human monocyte U937 cell cycle arrest through p53-independent p21 pathway 

  14. Thioacetamide promotes osteoclast transformation of bone marrow macrophages by influencing PI3K/AKT pathways 

  15. Astaxanthin Prevents Tuberculosis-Associated Inflammatory Injury by Inhibiting the Caspase 4/11-Gasdermin-Pyroptosis Pathway 

  16. The long noncoding RNA H19 promotes tamoxifen resistance in breast cancer via autophagy 

  17. Akt3 is a target of miR-29c-3p and serves an important function in the pathogenesis of congenital heart disease 

  18. Transcription Factor SOX18 Promotes Clear Cell Renal Cell Carcinoma Progression and Alleviates Cabozantinib-Mediated Inhibitory Effects 

  19. Characterization of the release and biological significance of cell-free DNA from breast cancer cell lines 

  20. WFDC2 suppresses prostate cancer metastasis by modulating EGFR signaling inactivation 

  21. MicroRNA-141 inhibits tumor growth and minimizes therapy resistance in colorectal cancer 

  22. Hydroxychloroquine sensitizes chronic myeloid leukemia cells to Vγ9Vδ2 T cell-mediated lysis independent of autophagy 

  23. S100P is Overexpressed in Squamous Cell and Adenosquamous Carcinoma Subtypes of Endometrial Cancer and Promotes Cancer Cell Proliferation and Invasion 

  24. Exosomes derived from miR-126-3p-overexpressing synovial fibroblasts suppress chondrocyte inflammation and cartilage degradation in a rat model of osteoarthritis 

  25. Resveratrol Suppresses Growth and Migration of Myelodysplastic Cells by Inhibiting the Expression of Elevated Cyclin D1 (CCND1) 

  26. MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis 

  27. MicroRNA?1 affects the development of the neural crest and craniofacial skeleton via the mitochondrial apoptosis pathway 

  28. STAT3 stimulates adipogenic stem cell proliferation and cooperates with HMGA2 during the early stage of differentiation to promote adipogenesis 

  29. Low expression of long noncoding RNA CASC2 indicates a poor prognosis and promotes tumorigenesis in thyroid carcinoma 

  30. MicroRNA?328 directly targets p21?activated protein kinase 6 inhibiting prostate cancer proliferation and enhancing docetaxel sensitivity 

  31. Spliceosome-associated factor CTNNBL1 promotes proliferation and invasion in ovarian cancer 

  32. Knockdown of miR-629 Inhibits Ovarian Cancer Malignant Behaviors by Targeting Testis-Specific Y-Like Protein 5 

  33. miRNA-30a functions as a tumor suppressor by downregulating cyclin E2 expression in castration-resistant prostate cancer 

  34. Clinical, cellular, and bioinformatic analyses reveal involvement of WRAP53 overexpression in carcinogenesis of lung adenocarcinoma 

  35. Downregulation of RUVBL1 inhibits proliferation of lung adenocarcinoma cells by G1/S phase cell cycle arrest via multiple mechanisms 

  36. A novel lncRNA TCLlnc1 promotes peripheral T cell lymphoma progression through acting as a modular scaffold of HNRNPD and YBX1 complexes 

  37. Clinical Significance of Long Non-coding RNA MALAT1 Expression in Tissue and Serum of Breast Cancer 

  38. Long noncoding RNA FEZF1-AS1 indicates a poor prognosis of gastric cancer and promotes tumorigenesis via activation of Wnt signaling pathway 

  39. Upregulation of long non-coding RNA HOXA-AS2 promotes proliferation and induces epithelial-mesenchymal transition in gallbladder carcinoma 

  40. E3 ubiquitin ligase RNF126 affects bladder cancer progression through regulation of PTEN stability 

  41. Shikonin overcomes drug resistance and induces necroptosis by regulating the miR-92a-1-5p/MLKL axis in chronic myeloid leukemia 

  42. THBS2 is a Potential Prognostic Biomarker in Colorectal Cancer 

  43. Generation of affibody molecules specific for HPV16 E7 recognition 

  44. Neferine inhibits proliferation and collagen synthesis induced by high glucose in cardiac fibroblasts and reduces cardiac fibrosis in diabetic mice 

  45. Functional mechanism of the enhancement of 5?fluorouracil sensitivity by TUSC4 in colon cancer cells 

  46. Hsa_circ_0008934 promotes the proliferation and migration of osteosarcoma cells by targeting miR-145-5p to enhance E2F3 expression 

  47. MiR-1231 correlates tumor prognosis and inhibits cell growth in ovarian cancer 

  48. Ganoderic acid D induces synergistic autophagic cell death except for apoptosis in ESCC cells 

  49. ANLN, Regulated by SP2, Promotes Colorectal Carcinoma Cell Proliferation via PI3K/AKT and MAPK Signaling Pathway 

  50. 12-Chloracetyl-PPD, a novel dammarane derivative, shows anti-cancer activity via delay the progression of cell cycle G2/M phase and reactive oxygen species-mediate cell apoptosis 

  51. Targeting hexokinase 2 increases the sensitivity of oxaliplatin by Twist1 in colorectal cancer 

  52. Fucoidan inhibits EGFR redistribution and potentiates sorafenib to overcome sorafenib-resistant hepatocellular carcinoma 

  53. Artemisitene suppresses rheumatoid arthritis progression via modulating METTL3-mediated N6-methyladenosine modification of ICAM2?mRNA in fibroblast-like synoviocytes 

  54. Retinoic acid receptor gamma is required for proliferation of pancreatic cancer cells 

  55. Alantolactone induces concurrent apoptosis and GSDME-dependent pyroptosis of anaplastic thyroid cancer through ROS mitochondria-dependent caspase pathway 

  56. Calcilytic NPS2143 promotes proliferation and inhibits apoptosis of spontaneously hypertensive rat vascular smooth muscle cells via activation of the renin?angiotensin system 

  57. Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process 

  58. STRA6 exerts oncogenic role in gastric tumorigenesis by acting as a crucial target of miR-873 

  59. High expression of G-protein signaling modulator 2 in hepatocellular carcinoma facilitates tumor growth and metastasis by activating the PI3K/AKT signaling pathway 

  60. Xylopins A–F, six rare guaiane dimers with three different connecting modes from Xylopia vielana 

  61. Vieloplains A-G, seven new guaiane-type sesquiterpenoid dimers from Xylopia vielana 

  62. S100A16 suppresses the growth and survival of leukaemia cells?and correlates with relapse and relapse free survival in adults with Philadelphia chromosome-negative B-cell acute lymphoblastic leukaemia 

  63. miR-363-3p inhibits tumor growth by targeting PCNA in lung adenocarcinoma 

  64. Overexpression of CXCL2 inhibits cell proliferation and promotes apoptosis in hepatocellular carcinoma 

  65. CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis 

  66. PADI6 Regulates Trophoblast Cell Migration-Invasion Through the Hippo/YAP1 Pathway in Hydatidiform Moles 

  67. Long non-coding RNA00844 inhibits MAPK signaling to suppress the progression of hepatocellular carcinoma by targeting AZGP1 

  68. The resistant effect of SIRT1 in oxidative stress-induced senescence of rat nucleus pulposus cell is regulated by Akt-FoxO1 pathway 

  69. PARK2 Suppresses Proliferation and Tumorigenicity in Non-small Cell Lung Cancer 

  70. Histone H3K36me2 demethylase KDM2A promotes bladder cancer progression through epigenetically silencing RARRES3 

  71. Liver-enriched activator protein 1 as an isoform of CCAAT/enhancer-binding protein beta suppresses stem cell features of hepatocellular carcinoma 

  72. AKAP9 Upregulation Predicts Unfavorable Prognosis in Pediatric Acute Myeloid Leukemia and Promotes Stemness Properties via the Wnt/β-Catenin Pathway 

  73. Clock Gene Bmal1 Disruption in Vascular Smooth Muscle Cells Worsens Carotid Atherosclerotic Lesions 

  74. TAF?Iβ deficiency inhibits proliferation and promotes apoptosis by rescuing PP2A and inhibiting the AKT/GSK?3β pathway in leukemic cells 

  75. BCAP31, a cancer/testis antigen-like protein, can act as a probe for non-small-cell lung cancer metastasis 

  76. Sustained release of Ganoderma lucidum antitumor drugs using a sandwich structured material prepared by electrospinning 

  77. Preclinical efficacy and involvement of mTOR signaling in the mechanism of Orf virus against nasopharyngeal carcinoma cells 

  78. miR-182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro 

  79. Immunomodulatory effect of thymopentin on lymphocytes from supramammary lymph nodes of dairy cows 

  80. Pinin promotes tumor progression via activating CREB through PI3K/AKT and ERK/MAPK pathway in prostate cancer 

  81. Hypoxia Stimulates SUMOylation-Dependent Stabilization of KDM5B 

  82. HN1L is essential for cell growth and survival during nucleopolyhedrovirus infection in silkworm, Bombyx mori 

  83. Pifithrin-μ is efficacious against non-small cell lung cancer via inhibition of heat shock protein 70 

  84. LncRNA882 regulates leukemia inhibitory factor (LIF) by sponging miR-15b in the endometrial epithelium cells of dairy goat 

  85. Blockade of the mTOR signaling pathway with rapamycin ameliorates aristolochic acid nephropathy 

  86. Characterization and anti-uterine tumor effect of extract from Prunella vulgaris L. 

  87. Therapeutic potential of transplanted placental mesenchymal stem cells in treating Chinese miniature pigs with acute liver failure 

  88. The miR155HG/miR-185/ANXA2 loop contributes to glioblastoma growth and progression 

  89. Long non-coding RNA MACC1-AS1 promoted pancreatic carcinoma progression through activation of PAX8/NOTCH1 signaling pathway 

  90. MiR-206 suppresses the deterioration of intrahepatic cholangiocarcinoma and promotes sensitivity to chemotherapy by inhibiting interactions with stromal CAFs 

  91. Alkaloid Dimers Isolated from Thalictrum baicalense Have Antitumor Activities 

  92. Effect of ximenynic acid on cell cycle arrest and apoptosis and COX-1 in HepG2 cells 

  93. Cuprous oxide nanoparticles trigger ER stress-induced apoptosis by regulating copper trafficking and overcoming resistance to sunitinib therapy in renal cancer 

  94. N7-methylguanosine tRNA modification promotes tumorigenesis and chemoresistance through WNT/β-catenin pathway in nasopharyngeal carcinoma 

  95. Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells. 

  96. TRIP13 interference inhibits the proliferation and metastasis of thyroid cancer cells through regulating TTC5/p53 pathway and epithelial-mesenchymal transition related genes expression 

  97. The Combined Use of Orf Virus and PAK4 Inhibitor Exerts Anti-tumor Effect in Breast Cancer 

  98. Bone marrow mesenchymal stromal cells affect the cell cycle arrest effect of genotoxic agents on acute lymphocytic leukemia cells via p21 down-regulation 

  99. Polystyrene micro- and nano-particle coexposure injures fetal thalamus by inducing ROS-mediated cell apoptosis 

  100. Curcusone C induces telomeric DNA-damage response in cancer cells through inhibition of telomeric repeat factor 2 

  101. The effects of SAHA on radiosensitivity in pancreatic cancer cells by inducing apoptosis and targeting RAD51 

  102. miR-148a-3p regulates proliferation and apoptosis of bovine muscle cells by targeting KLF6 

  103. ΔFosB regulates rosiglitazone-induced milk fat synthesis and cell survival 

  104. A MYBL2 complex for RRM2 transactivation and the synthetic effect of MYBL2 knockdown with WEE1 inhibition against colorectal cancer 

  105. Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines 

  106. PMEPA1 induces EMT via a non-canonical TGF-β signalling in colorectal cancer 

  107. Prometryn induces apoptotic cell death through cell cycle arrest and oxidative DNA damage 

  108. Design, synthesis, and bioactivity study on Lissodendrins B derivatives as PARP1 inhibitor 

  109. Immunosuppressive activity of pogostone on T cells: Blocking proliferation via S phase arrest 

  110. The YY1/miR-548t-5p/CXCL11 signaling axis regulates cell proliferation and metastasis in human pancreatic cancer 

  111. Baicalein inhibits the growth of oral squamous cell carcinoma cells by downregulating the expression of transcription factor Sp1 

  112. miR-378a-3p promotes differentiation and inhibits proliferation of myoblasts by targeting HDAC4 in skeletal muscle development 

  113. circFGFR4 Promotes Differentiation of Myoblasts via Binding miR-107 to Relieve Its Inhibition of Wnt3a 

  114. MiR-520d-3p antitumor activity in human breast cancer via post-transcriptional regulation of spindle and kinetochore associated 2 expression 

  115. The novel long noncoding RNA u50535 promotes colorectal cancer growth and metastasis by regulating CCL20 

  116. Long non?coding RNA maternally expressed gene?3 affects cell proliferation, apoptosis and migration by targeting the microRNA?9?5p/midkine axis and activating the phosphoinositide?dependent kinase/AKT pathway in hepatocellular carcinoma 

  117. Long non-coding RNA AC026166.2-001 inhibits cell proliferation and migration in laryngeal squamous cell carcinoma by regulating the miR-24-3p/p27 axis 

  118. miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression 

  119. SQLE Promotes Differentiation and Apoptosis of Bovine Skeletal Muscle-Derived Mesenchymal Stem Cells 

  120. MiR-449a regulates caprine endometrial stromal cell apoptosis and endometrial receptivity 

1
Liu Y, Li Q, Gu M, et al. A Second Near-Infrared Ru(II) Polypyridyl Complex for Synergistic Chemo-Photothermal Therapy[J]. J. Med. Chem., https://doi.org/10.1021/acs.jmedchem.1c01736

查看原文
2
Yan, Y., Zhang, Y., Li, M., Zhang, Y., Zhang, X., Zhang, X., Xu, Y., Wei, W., Wang, J., Xu, X., et al. (2021). C644-0303, a small-molecule inhibitor of the Wnt/β-catenin pathway, suppresses colorectal cancer growth. Cancer Science 112, 4722-4735. IF 6.713

查看原文
3
Du Jing,Wang Xu,Li Yanchun et al. DHA exhibits synergistic therapeutic efficacy with cisplatin to induce ferroptosis in pancreatic ductal adenocarcinoma via modulation of iron metabolism.[J] .Cell Death Dis, 2021, 12: 705.

查看原文
4
Wang K J , Wang K Y , Zhang H Z , et al. Up-Regulation of RIP3 Alleviates Prostate Cancer Progression by Activation of RIP3/MLKL Signaling Pathway and Induction of Necroptosis[J]. Frontiers in Oncology, 2020, 10:1720.(IF=4.848)

查看原文
5
Ye S , Xu M , Zhu T , et al. Cytoglobin promotes sensitivity to ferroptosis by regulating p53℡AP1 axis in colon cancer cells[J]. Journal of Cellular and Molecular Medicine, 2021.

查看原文
6
Xiaojiao Zheng,Kejun Xu, et al. MiR-486-5p Act as a Biomarker in Endometrial Carcinoma: Promotes Cell Proliferation, Migration, Invasion by Targeting MARK1[J].OncoTargets and Therapy, 2020:13 4843–4853 (IF:3.337)

查看原文
7
Xiang-Yu Meng, Hui-Zhi Zhang, Yi-Yue Ren, Ke-Jie Wang, Jun-Feng Chen, Rui Su, Jun-Hui Jiang, Ping Wang, Qi Ma. Pinin promotes tumor progression via activating CREB through PI3K/AKT and ERK/MAPK pathway in prostate cancer[J]. Am J Cancer Res, 2021,11(4):1286-1303.(IF:5.177)

查看原文
9
Jing Du#, Yi Zhou#, Yanchun Li#, Jun Xia, Yongjian Chen, Sufeng Chen, Xin Wang, Weidong Sun, Tongtong Wang, Xueying Ren, Xu Wang, Yihan An, Kang Lu, Wanye Hu, Siyuan Huang, Jianghui Li, Xiangmin Tong*, Ying Wang*,Identification of Frataxin as a regulator of ferroptosis, Redox Biology, 32 (2020), doi.org/10.1016/j.redox.2020.101483. (IF:9.98)

查看原文
10
L Liu, H Xie, X Chen, et al. Differential response of normal human epidermal keratinocytes and HaCaT cells to hydrogen peroxide-induced oxidative stress[J]. CLIN EXP DERMATOL, 2011, 37: 772-780 (IF:1.198)

查看原文
16
MiersalijiangYascn, QinmingFei, JianZhang, et al. Changes of number of cells expressing proliferation and progenitor cell markers with age in rabbit intervertebral discs[J]. ACTA BIOCH BIOPH SIN, 2013, 45(5): 368-376 (IF:1.807)

查看原文
17
W Li, S Liu, Y Chen, et al. Effects of the dilution rate on cell cycle distribution and PEI-mediated transient gene expression by CHO cells in continuous culture[J]. PROCESS BIOCHEM, 2013, 48: 1572-1580 (IF:2.414)

查看原文
18
Qian C , Yao J , Wang J , et al. ERK1/2 inhibition enhances apoptosis induced by JAK2 silencing in human gastric cancer SGC7901 cells[J]. Molecular and Cellular Biochemistry, 2014, 387(1-2):159-170 (IF:2.329)

查看原文
19
Bing Xiu, Wenjun Zhang, Binbin Huang, et al. Genetic inhibition of vascular endothelial growth factor receptor-1 significantly inhibits the migration and proliferation of leukemia cells and increases their sensitivity to chemotherapy[J]. ONCOL REP, 2013, 29: 2030-2038 (IF:2.297)

查看原文
20
Xiao Yang, Ya-ping Wang, Feng-xiang Liu, et al. Increased invasiveness of osteosarcoma mesenchymal stem cells induced by bone-morphogenetic protein-2[J]. IN VITRO CELL DEV-AN, 2013, 49: 2278 (IF:1.289)

查看原文
21
Jing Lua, Guoren Huang, Shuang Zhang, et al. The inhibition of 2,3-dichloro-1-propanol on T cell in vitro and in vivo[J]. INT IMMUNOPHARMACOL, 2013, 17: 321-328 (IF:2.417)

查看原文
22
Wang J, Chen X, Zhou Z, et al. The Inhibitory Effect of 3β-Hydroxy-12-oleanen-27-oic Acid on Growth and Motility of Human Hepatoma HepG2 Cells through JNK and Akt Signaling Pathway[J]. Evidence-Based Complementary and Alternative Medicine, 2013, 2013:1-17 (IF:1.722)

查看原文
23
Li T, Zhang Q, Zhang J, et al. Fenofibrate induces apoptosis of triple-negative breast cancer cells via activation of NF-κB pathway[J]. BMC Cancer, 2014, 14(1): 96 (IF:3.333)

查看原文
24
Jiefang Xia, Zhuanzi Wang, Qingfang Liu, et al. The role of autophagy in cell survival from heavy ion irradiation in the plateau region[J]. Nuclear Science and Techniques, 2014, 25(3): 32-37 (IF:0.088)

查看原文
25
RY Huang, SS Li, HZ Guo, et al. Thromboxane A2 exerts promoting effects on cell proliferation through mediating cyclooxygenase-2 signal in lung adenocarcinoma cells[J]. J Cancer Res Clin Oncol, 2014, 140(3): 375-86 (IF:2.914)

查看原文
34
Zhang Xue, Bai Jian, Wu Cheng-lin, et al. Candidaglabrata induced infection of rat tracheal epithelial cells is mediated by TLR-2 induced activation of NF-κB[J]. Microbial Pathogenesis, 2016, 91: 115-122 (IF:1.794)

查看原文
35
Jiang Y, Zhang L. Mechanism of all-transretinoic acid increasing retinoblastoma sensitivity to vincristine[J]. Asian Pacific journal of tropical medicine, 2016, 9(3): 278-282 (IF:0.841)

查看原文
36
Li L, Wu J J, Zheng F, et al. Inhibition of EZH2 via activation of SAPK/JNK and reduction of p65 and DNMT1 as a novel mechanism in inhibition of human lung cancer cells by polyphyllin I[J]. Journal of Experimental & Clinical Cancer Research, 2016, 35(1): 112 (IF:4.357)

查看原文
37
Liu L, Wang D, Li L, et al. Dehydroepiandrosterone inhibits cell proliferation and improves viability by regulating S phase and mitochondrial permeability in primary rat Leydig cells[J]. Molecular?Medicine?Reports, 2016, 14: 705-714 (IF:1.559)

查看原文
38
Gu Y, Wang Y, Zhou Q, et al. Inhibition of Nickel Nanoparticles-Induced Toxicity by Epigallocatechin-3-Gallate in JB6 Cells May Be through Down-Regulation of the MAPK Signaling Pathways[J]. PLOS ONE, 2016, 11(3): e0150954 (IF:3.057)

查看原文
39
Quyang Q, Zhang L, Jiang Y, et al. The membrane complement regulatory protein CD59 promotes tumor growth and predicts poor prognosis in breast cancer[J]. International Journal of Oncology, 2016, 48(5): 2015-2024 (IF:3.018)

查看原文
40
Yan Y, Xie M, Zhang L, et al. ras-related associated with diabetes gene acts as a suppressor and inhibits Warburg effect in hepatocellular carcinoma[J]. oncotargets and therapy, 2016, 9: 3925-3937 (IF:2.272)

查看原文
41
Chen Y, Huang L, Cai J, et al. Down-regulation of BubR1 inhibits growth and induces apoptosis in hepatitis B virus-related hepatocellular carcinoma cells[J]. International Journal of?Clinical?and Experimental Pathology, 2016, 9(3): 4129-4139 (IF:1.893)

查看原文
42
Liu W, Wang J, Wang L, et al. Ras-association domain family 10 acts as a novel tumor suppressor through modulating MMP2 in hepatocarcinoma[J]. Oncogenesis, 2016, 5(6): e237 (IF:5.021)

查看原文
43
Hu S, Zhang M, Sun F, et al. MiR-375 controls porcine pancreatic stem cell fate by targeting 3-phosphoinositide-dependent protein kinase-1 (Pdk1)[J]. Cell Proliferation, 2016, 49(3): 395-406 (IF:3.084)

查看原文
44
Liu AJ, Du BY, Yi H, et al. Loureirin A Activates Wnt/β-Catenin Pathway to Promote Wound with Follicle Stem Cell-Seeded Tissue-Engineered Skin Healing[J]. Journal of Biomaterials and Tissue Engineering, 2016, 6: 427-432 (IF:1.287)

查看原文
45
Lan Sun, Huaibao Zhang, Laixi Bi, et al. Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice[J]. Am J Transl Res, 2016, 8(5): 2011-2021 (IF:3.146)

查看原文
46
Ni B, Hu J, Chen D, et al. Alternative splicing of spleen tyrosine kinase differentially regulates colorectal cancer progression[J]. Oncology Letters, 2016 (IF:1.482)

查看原文
47
Li N, Mu H, Zheng L, et al. EIF2S3Y suppresses the pluripotency state and promotes the proliferation of mouse embryonic stem cells[J]. Oncotarget, 2016, 7(10): 11321-11331 (IF:5.008)

查看原文
48
Peng P, Wu W, Zhao J, et al. Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer[J]. Scientific Reports, 2016, 6: 29604 (IF:5.228)

查看原文
49
Zhao J, Shu P, Duan F, et al. Loss of OLFM4 promotes tumor migration through inducing interleukin-8 expression and predicts lymph node metastasis in early gastric cancer[J]. Oncogenesis, 2016, 5(6): e234 (IF:5.021)

查看原文
50
Zhou Y, Ma J, Zhang J, et al. Pifithrin-μ is efficacious against non-small cell lung cancer via inhibition of heat shock protein 70[J]. Oncology Reports, 2016 (IF:2.662)

查看原文
51
Su Z Q, Liu Y H, Guo H Z, et al. Effect-enhancing and toxicity-reducing activity of usnic acid in ascitic tumor-bearing mice treated with bleomycin[J]. International Immunopharmacology, 2017, 46: 146-155 (IF:2.551)

查看原文
52
He X Q, Zhang Y F, Yu J J, et al. High expression of G-protein signaling modulator 2 in hepatocellular carcinoma facilitates tumor growth and metastasis by activating the PI3K/AKT signaling pathway[J]. Tumor Biology, 2017, 39(3): 101042831769597 (IF:2.926)

查看原文
53
Sufen F, Jinhua Y, Qing S, et al. Downregulation of UBC9 promotes apoptosis of activated human LX-2 hepatic stellate cells by suppressing the canonical NF-κB signaling pathway[J]. PLOS ONE, 2017, 12(3): e0174374 (IF:3.234)

查看原文
54
Yao G L, Pan C F, Xu H, et al. Long noncoding RNA RP11-766N7.4 functions as a tumor suppressor by regulating epithelial-mesenchymal transition in esophageal squamous cell carcinoma[J]. Biomedicine & Pharmacotherapy, 2017, 88: 778-785 (IF:2.7)

查看原文
55
Qin X, Chen J, Wu L, et al. MiR-30b-5p acts as a tumor suppressor, repressing cell proliferation and cell cycle in human hepatocellular carcinoma[J]. Biomedicine & Pharmacotherapy, 2017, 89: 742-750 (IF:2.7)

查看原文
56
Weining Wu, Xu Zhou, Tianfu Yu, et al. The malignancy of miR-18a in human glioblastoma via directly targeting CBX7[J]. Am J Cancer Res, 2017, 7(1):64-76 (IF:3.968)

查看原文
57
Zhou W, Wang S, Ying Y, et al. MiR-196b/miR-1290 participate in the antitumor effect of resveratrol via regulation of IGFBP3 expression in acute lymphoblastic leukemia[J]. Oncology Reports, 2016 (IF:2.662)

查看原文
58
Wang Y, Chen T, Huang H, et al. MiR-363-3p inhibits tumor growth by targeting PCNA in lung adenocarcinoma[J]. Oncotarget, 2017, 8(12): 20133-20144 (IF:5.008)

查看原文
59
Xu Y, Zheng Y, Liu H, et al. Modulation of IGF2BP1 by long non-coding RNA HCG11 suppresses apoptosis of hepatocellular carcinoma cells via MAPK signaling transduction[J]. International Journal of Oncology, 2017, 51(3): 791-800 (IF:2.267)

查看原文
60
Hui Song, Ping He, Tiansong Shao, et al. Long non-coding RNA XIST functions as an oncogene in human colorectal cancer by targeting miR-132-3p[J]. JBUON, 2017, 22(3): 696-703 (IF:1.344)

查看原文
61
Wang W, Kong P, Ma G, et al. Characterization of the release and biological significance of cell-free DNA from breast cancer cell lines[J]. Oncotarget, 2017, 8(26): 43180-43191 (IF:5.168)

查看原文
62
Cai J, Liu T, Jiang X, et al. Downregulation of USP18 inhibits growth and induces apoptosis in hepatitis B virus-related hepatocellular carcinoma cells by suppressing BCL2L1[J]. Experimental Cell Research, 2017: S0014482717303609 (IF:3.378)

查看原文
63
Ma L Y, Xie X W, Ma L, et al. Downregulated long non-coding RNA TRPM2-AS inhibits cisplatin resistance of non-small cell lung cancer cells via activation of p53- p66shc pathway[J]. Eur Rev Med Pharmacol Sci, 2017, 21(11): 2626-2634 (IF:1.779)

查看原文
64
Lou G, Yang Y, Liu F, et al. MiR-122 modification enhances the therapeutic efficacy of adipose tissue-derived mesenchymal stem cells against liver fibrosis[J]. Journal of Cellular and Molecular Medicine, 2017 (IF:4.302)

查看原文
65
Wu J, Hu G, Dong Y, et al. Matrine induces Akt/mTOR signalling inhibition-mediated autophagy and apoptosis in acute myeloid leukaemia cells[J]. Journal of Cellular and Molecular Medicine, 2016 (IF:4.397)

查看原文
66
Li A, Li J, Lin J, et al. COL11A1 is overexpressed in gastric cancer tissues and regulates proliferation, migration and invasion of HGC-27 gastric cancer cells in vitro[J]. Oncology Reports, 2017, 37(1): 333-340 (IF:2.662)

查看原文
67
Wang Y, Qin X, Guo T, et al. Up-regulation of CDK16 by multiple mechanisms in hepatocellular carcinoma promotes tumor progression[J]. Journal of Experimental & Clinical Cancer Research, 2017, 36(1): 97 (IF:5.189)

查看原文
68
Yao G L, Pan C F, Xu H, et al. Long noncoding RNA RP11-766N7.4 functions as a tumor suppressor by regulating epithelial-mesenchymal transition in esophageal squamous cell carcinoma[J]. Biomedicine & Pharmacotherapy, 2017, 88: 778-785 (IF:2.759)

查看原文
69
Wang H, Li S, Luo X, et al. Knockdown of PARP6 or survivin promotes cell apoptosis and inhibits cell invasion of colorectal adenocarcinoma cells[J]. Oncology Reports, 2017 (IF:2.662)

查看原文
70
Du Y, Wang Q, Zhang X, et al. Lysophosphatidylcholine acyltransferase 1 upregulation and concomitant phospholipid alterations in clear cell renal cell carcinoma[J]. J Exp Clin Cancer Res, 2017, 36(1): 66 (IF:5.189)

查看原文
71
Wang C, Su Z, Hou H, et al. Inhibition of Anaphase-Promoting Complex by Silence APC/CCdh1to Enhance Radiosensitivity of Nasopharyngeal Carcinoma Cells[J]. Journal of Cellular Biochemistry, 2016 (IF:3.446)

查看原文
72
Lu S, et al. MiRNA?186 inhibits prostate cancer cell proliferation and tumor growth by targeting YY1 and CDK6[J]. Experimental & Therapeutic Medicine, 2017, 13(6): 3309-3314 (IF:1.261)

查看原文
73
Zhi T, Jiang K, Zhang C, et al. MicroRNA-1301 inhibits proliferation of human glioma cells by directly targeting N-Ras[J]. American Journal of Cancer Research, 2017, 7(4): 982-998 (IF:3.998)

查看原文
74
Zhang F, Ren H, Shao X, et al. Preservation media, durations and cell concentrations of short-term storage affect key features of human adipose-derived mesenchymal stem cells for therapeutic application[J]. PeerJ, 2017, 5(6): e3301 (IF:2.177)

查看原文
75
Xia C, Shui L, Lou G, et al. 0404 inhibits hepatocellular carcinoma through a p53/miR-34a/SIRT1 positive feedback loop[J]. Scientific Reports, 2017, 7(1): 4396 (IF:4.259)

查看原文
76
Xie P, Yang J P, Cao Y, et al. Promoting tumorigenesis in nasopharyngeal carcinoma, NEDD8 serves as a potential theranostic target[J]. Cell Death and Disease, 2017, 8(6): e2834 (IF:5.965)

查看原文
77
Liu T, Zhao M, Liu J, et al. Tumor suppressor bromodomain-containing protein 7 cooperates with Smads to promote transforming growth factor-β responses[J]. Oncogene, 2016 (IF:7.519)

查看原文
78
Yang Q, Wang Y, Yang Q, et al. Cuprous oxide nanoparticles trigger ER stress-induced apoptosis by regulating copper trafficking and overcoming resistance to sunitinib therapy in renal cancer[J]. Biomaterials, 2017, 146: 72-85 (IF:8.402)

查看原文
79
Wang M, Cao J, Zhu J Y, et al. Curcusone C induces telomeric DNA-damage response in cancer cells through inhibition of telomeric repeat factor 2[J]. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 2017, 1865(11): 1372-1382 (IF:2.747)

查看原文
80
Zhang Y, Zeng D, Cao J, et al. Interaction of Quindoline derivative with telomeric repeat-containing RNA induces telomeric DNA-damage response in cancer cells through inhibition of telomeric repeat factor 2[J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2017: S0304416517302994 (IF:4.702)

查看原文
81
Zhang J, Zhang J, Zhang J, et al. MicroRNA-625 inhibits the proliferation and increases the chemosensitivity of glioma by directly targeting AKT2[J]. American Journal of Cancer Research, 2017, 7(9): 1835-1849 (IF:6.072)

查看原文
82
Xu F, Chang K, Ma J, et al. The Oncogenic Role of COL23A1 in Clear Cell Renal Cell Carcinoma[J]. Scientific Reports, 2017, 7(1): 9846 (IF:4.259)

查看原文
83
Fen-Fen C, Yan X, Ying P, et al. MiR-425-5p Inhibits Differentiation and Proliferation in Porcine Intramuscular Preadipocytes[J]. International Journal of Molecular Sciences, 2017, 18(10): 2101 (IF:3.226)

查看原文
84
Yang S, Sheng L, Xu K, et al. Anticancer effect of quinacrine on diffuse large B?cell lymphoma via inhibition of MSI2?NUMB signaling pathway[J]. Molecular Medicine Reports, 2017 (IF:1.692)

查看原文
85
Li H, Yang J, Wei X, et al. CircFUT10 reduces proliferation and facilitates differentiation of myoblasts by sponging miR-133a[J]. Journal of Cellular Physiology, 2017 (IF:4.08)

查看原文
86
Xiu-Yi L, Liang M, Jun-Feng C, et al. Knockdown of DUXAP10 inhibits proliferation and promotes apoptosis in bladder cancer cells via PI3K/Akt/mTOR signaling pathway[J]. International Journal of Oncology, 2017 (IF:3.079)

查看原文
87
Wei X, Li H, Yang J, et al. Circular RNA profiling reveals an abundant circLMO7 that regulates myoblasts differentiation and survival by sponging miR-378a-3p[J]. Cell Death and Disease, 2017, 8(10): e3153 (IF:5.965)

查看原文
88
Zhou H, Zheng L, Lu K, et al. Downregulation of Cohesin Loading Factor Nipped-B-Like Protein (NIPBL) Induces Cell Cycle Arrest, Apoptosis, and Autophagy of Breast Cancer Cell Lines[J]. Medical Science Monitor International Medical Journal of Experimental & Clinical Research, 2017, 23: 4817 (IF:0.799)

查看原文
89
Zheng Jing, Li Huiling, Xu Dongting, et al. Upregulation of Tyrosine Kinase FYN in Human Thyroid Carcinoma: Role in Modulating Tumor Cell Proliferation, Invasion, and Migration [J]. Cancer Biotherapy and Radiopharmaceuticals, 2017, 32(9): 320-326 (IF:1.689)

查看原文
90
Gaoxiao X, Saixing D, Jianye H, et al. GosB Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells[J]. BioMed Research International, 2017, 2017: 1-12 (IF:2.476)

查看原文
91
Zhang F, Ren H, Shao X, et al. Preservation media, durations and cell concentrations of short-term storage affect key features of human adipose-derived mesenchymal stem cells for therapeutic application[J]. PeerJ, 2017, 5(6): e3301 (IF:)

查看原文
92
Xia C, Shui L, Lou G, et al. 0404 inhibits hepatocellular carcinoma through a p53/miR-34a/SIRT1 positive feedback loop[J]. Scientific Reports, 2017, 7(1): 4396 (IF:4.122)

查看原文
93
Xie P, Yang J P, Cao Y, et al. Promoting tumorigenesis in nasopharyngeal carcinoma, NEDD8 serves as a potential theranostic target[J]. Cell Death and Disease, 2017, 8(6): e2834 (IF:5.638)

查看原文
94
Zhou X, Wu W, Zeng A, et al. MicroRNA-141-3p promotes glioma cell growth and temozolomide resistance by directly targeting p53[J]. Oncotarget, 2017, 8(41) (IF:1.39)

查看原文
95
Liu T, Zhao M, Liu J, et al. Tumor suppressor bromodomain-containing protein 7 cooperates with Smads to promote transforming growth factor-β responses[J]. Oncogene, 2016 (IF:7.519)

查看原文
96
Li H, Yang J, Wei X, et al. CircFUT10 reduces proliferation and facilitates differentiation of myoblasts by sponging miR-133a[J]. Journal of Cellular Physiology, 2017 (IF:3.923)

查看原文
97
Wei X, Li H, Zhao G, et al. ΔFosB regulates rosiglitazone-induced milk fat synthesis and cell survival[J]. Journal of Cellular Physiology, 2017 (IF:3.923)

查看原文
98
Huang C, Luo H. miR-19-5p Enhances Tumorigenesis in Human Colorectal Cancer Cells by Targeting TSPYL5[J]. Dna & Cell Biology, 2017, 37(1) (IF:2.236)

查看原文
99
Zhu H, Liu Q, Tang J, et al. Alpha1-ACT Functions as a Tumour Suppressor in Hepatocellular Carcinoma by Inhibiting the PI3K/AKT/mTOR Signalling Pathway via Activation of PTEN[J]. Cell Physiol Biochem, 2017, 41: 2289-2306 (IF:5.55)

查看原文
100
Lingling S, Ting W, Dongdong T, et al. Prolyl hydroxylases positively regulated LPS-induced inflammation in human gingival fibroblasts via TLR4/MyD88-mediated AKT/NF-κB and MAPK pathways[J]. Cell Proliferation, 2018: e12516 (IF:5.039)

查看原文
101
Fang-Lin Z, Jin-Ling C, Hong-Yan X, et al. Cancer-associated MORC2 mutant M276I regulates an hnRNPM-mediated CD44 splicing switch to promote invasion and metastasis in triple-negative breast cancer[J]. Cancer Research, 2018 (IF:8.556)

查看原文
102
Wei L, Lin W, Jing Y, et al. Inhibition of C5a prevents IL-1β-induced alternations in rat synoviocytes, in vitro[J]. Molecular and Cellular Probes, 2018 (IF:1.689)

查看原文
103
Wenbo Z, Chao H, Aijun S, et al. Hydrogen alleviates cellular senescence via regulation of ROS/p53/p21 pathway in bone marrow-derived mesenchymal stem cells in vivo[J]. Biomedicine & Pharmacotherapy, 2018, 106: 1126-1134 (IF:3.743)

查看原文
104
Xiang S, Zou P, Wu J, et al. Crosstalk of NF-KB/P65 and LncRNA HOTAIR-Mediated Repression of MUC1 Expression Contribute to Synergistic Inhibition of Castration-Resistant Prostate Cancer by Polyphyllin 1-Enzalutamide Combination Treatment[J]. Cell Physiol Biochem, 2018, 47: 759-773 (IF:5.5)

查看原文
105
Ya-Ning W, Wu-Cai Y, Pei-Wei L, et al. Myocyte enhancer factor 2A promotes proliferation and its inhibition attenuates myogenic differentiation via myozenin 2 in bovine skeletal muscle myoblast[J]. PLOS ONE, 2018, 13(4): e0196255 (IF:2.806)

查看原文
106
Yong Z, Chun-Hua D. In vitro Effects of Nerve Growth Factor on Cardiac Fibroblasts Proliferation, Cell Cycle, Migration, and Myofibroblast Transformation[J]. Chinese Medical Journa, 2018, 131(7): 813-817 (IF:1.596)

查看原文
107
Li Y, Wei Y, Yuan W, et al. DNA damage induced by human CD40 ligand mutant promotes senescence and induces demethylation of GATA4 in lung cancer[J]. Oncology Reports, 2018 (IF:3.079)

查看原文
108
Wang J, Yang T, Chen H, et al. Oncogene RPA1 promotes proliferation of hepatocellular carcinoma via CDK4/Cyclin-D pathway[J]. Biochemical and Biophysical Research Communications, 2018, 498(3): 424-430 (IF:2.559)

查看原文
109
Yang J, Ren X, Zhang L, et al. Oridonin inhibits oral cancer growth and PI3K/Akt signaling pathway[J]. Biomedicine & pharmacotherapy, 2018, 100: 226 (IF:3.457)

查看原文
110
He X, Guan H, Liang W, et al. Exendin-4 modifies adipogenesis of human adipose-derived stromal cells isolated from omentum through multiple mechanisms[J]. International Journal of Obesity, 2018 (IF:4.042)

查看原文
111
Hanning Y, Zhiyao X, Mingming C, et al. Overexpression of Zwint predicts poor prognosis and promotes the proliferation of hepatocellular carcinoma by regulating cell-cycle-related proteins[J]. OncoTargets and Therapy, 2018, 11: 689-702 (IF:2.656)

查看原文
112
Ding G, Liu S, Ding Q, et al. Overexpression of HSD17B4 exerts tumor suppressive function in adrenocortical carcinoma and is not associated with hormone excess[J]. Oncotarget, 2017, 8(70): 114736-114745 (IF:5.168)

查看原文
113
He X, Wu C, Cui Y, et al. The aldehyde group of gossypol induces mitochondrial apoptosis via ROS-SIRT1-p53-PUMA pathway in male germline stem cell[J]. Oncotarget, 2017, 8(59) (IF:5.168)

查看原文
114
Lin Z, Lai S, He X, et al. Decreased long non-coding RNA MTM contributes to gastric cancer cell migration and invasion via modulating MT1F[J]. Oncotarget, 2017, 8(57): 97371-97383 (IF:5.168)

查看原文
115
Zheng J, Li H, Xu D, et al. Upregulation of Tyrosine Kinase FYN in Human Thyroid Carcinoma: Role in Modulating Tumor Cell Proliferation, Invasion, and Migration[J]. Cancer Biotherapy and Radiopharmaceuticals, 2017, 32(9): 320-326 (IF:1.682)

查看原文
121
Huang H, Zhang M, Zhang M, et al. Immune modulation of a novel lipid-soluble extract of, Pinellia pedatisecta, Schott in the tumor microenvironment of an HPV +, tumor-burdened mouse model[J]. Journal of Ethnopharmacology, 2018 (IF:3.115)

檢測步驟

文章目錄[隱藏]

一、可當天檢測的活細胞樣本

  1. 收集2 × 105- 1 × 106個細胞,離心棄上清。用PBS洗滌一次,離心棄上清。
  2. 加入1 mL DNA Staining Solution和10 μL Permeabilization Solution,渦旋振蕩5 - 10秒混勻。室溫避光孵育30分鐘。
  3. 選擇最低上樣速度,在流式細胞儀上進行檢測。如需在熒光顯微鏡下觀察,則離心細胞,棄上清,加入新鮮緩沖液重懸。滴加1滴細胞懸液至載玻片,蓋上蓋玻片后使用合適的濾光片進行觀察。

二、當天無法檢測的活細胞樣本

  1. 按照下述方法固定,或其他合適的方法固定
    • 收集2 × 105- 1 × 106個細胞,離心棄上清。輕彈管壁,使沉淀重懸在殘余的液體中,加入1 mL 室溫下的PBS。
    • 將細胞緩慢加入至3 mL無水乙醇(-20℃預冷)中,邊加邊高速攪拌。-20℃固定過夜,可保存數月。
  2. 檢測當天,將固定細胞離心,棄去乙醇,輕彈管壁使沉淀松散,加入2 - 5 mL 室溫下的PBS,放置15分鐘使細胞再次水化。離心,棄上清。
  3. 加入1 ml DNA Staining Solution,渦旋振蕩5 - 10秒混勻。室溫避光孵育30分鐘。
  4. 選擇最低上樣速度,在流式細胞儀上進行檢測。如需在熒光顯微鏡下觀察,則離心細胞,棄上清,加入新鮮緩沖液重懸。滴加1滴細胞懸液至載玻片,蓋上蓋玻片后使用合適的濾光片進行觀察。

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